Ultrasonography of the adrenal glands allows the description, biometric measurement and helps the diagnosis of diseases of the adrenal. Elastography is a promising ultrasound technique that evaluates tissue elasticity, such as compression, acoustic radiation force impulse (ARFI) and real-time shear velocity or supersonic (RSV). There are no reports of the use of this technique in the evaluation of the adrenal glands of animals and to describe the technique and to determine the quality and quantity (shear velocity) standards of ARFI elastography in evaluation of adrenal glands in dogs. Thirty healthy, adult dogs (60 adrenal in total) were selected. Echogenicity (hypoechoic, hyperechoic or mixed) and echotexture (homogeneous or heterogeneous), size and contours and margins (regular or not) were assessed by B-mode and using qualitative and quantitative elastography to detect the presence of deformities and tissue stiffness (elastogram) and shear velocities were obtained. Findings of ultrasonography were normal. Qualitative elastography shows that adrenal glands were not deformable and with homogeneous middle-grey areas. The results of shear wave velocity were similar statistically (p=0.3087): left adrenal - 1.42 m/s for cranial pole, 1.31 m/s for body region and 1.71 m/s for caudal pole; right adrenal - 1.42 m/s for cranial pole, 1.74 m/s for body region and 1.63 m/s for caudal pole. ARFI elastography of the adrenal glands in adult dogs was easily implemented and may provide baseline data in the study of this organ to allow the use of ARFI.