Vitreous loss is a serious complication of cataract surgery and is important to remove every trace of vitreous from the wound and anterior chamber. We retrospectively analyzed 22 cases of cataract extraction using phacoemulsification in whom vitreous loss occurred and automated anterior vitrectomy was performed. The dogs that underwent anterior vitrectomy were prospectively postoperatively followed for a minimum period of six months. All studies were in accordance with the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research. All patients included in this study received an ophthalmic examination. The surgery was performed by same surgeon using an Evolution phacoemulsification machine (U.S., IOL, Inc. Lexington, KY, USA). In all cases, phacoemulsification was performed before the anterior vitrectomy. The vitreous loss was defined as a discontinuation of the posterior capsule with the presence of vitreous in the capsular bag or anterior chamber at the time of surgery. Immediately after the vitreous loss was recognized the vitrectomy mode was selected in the console of the phacoemulsification. The bottle height was lowered to 15 to 20 centimeters above the eye. Anterior chamber was filled with viscoelastic. A vitreous cutter was inserted into the anterior chamber. Automated vitrectomy was performed by employing a monomanual technique. The cutter allowed the easy excision of nuclear fragments and sharp excision of the vitreous. Eighteen of the 22 operated eyes achieved intraocular pressure control in the early postoperative period, and this control was sustained throughout the course of follow up without antiglaucomatous medication. Two eyes developed an uncontrolled glaucoma leading to the enucleation. This study demonstrated that the automated anterior vitrectomy is a safe and effective way for excision of the lost vitreous during phacoemulsification in dogs.