Captive Brown Brocket Deer's Ocular Conjunctival Microflora (Mazama gouazoubira, Fischer, 1814)
World Small Animal Veterinary Association World Congress Proceedings, 2004
Martins, 1, Oriá, A. P.1, Souza, A. L. G.1, Schocken-Iturrino, R. P.2, Duarte, J. M. B.3, Laus, J. L.4
Graduate Student of Veterinary College, São Paulo State University, FCAV/UNESP, Jaboticabal, SP, Brazil1; DVM, PhD, Professor of Veterinary College, Department of Veterinary Pathology, São Paulo State University, FCAV/UNESP, Jaboticabal, SP, Brazil2; DVM, PhD, Professor of Veterinary College, Department of Animal Husbandry, São Paulo State University, FCAV/UNESP, Jaboticabal, SP, Brazil3; DVM, PhD, Professor of Veterinary College, Department of Medicine and Surgery, São Paulo State University, FCAV/UNESP, Jaboticabal, SP, Brazil4
The present study aimed to establish the normal ocular conjunctival microflora of captive brown brocket deer living in captivity in São Paulo State, Brazil.
Nine adult brown brocket deer (Mazama gouazoubira) from the Animal Husbandry Department at FCAV/UNESP were used on this study. The animals were manually retrained and the samples were collected using sterile cotton swabs that gently touched the inferior conjunctival sac, avoiding the contact with skin and eyelids. The samples were stored in tubes containing 5,0 mL of Brain Heart Infusion Broth (BHI) and incubated at 37°C for 18 - 48 hours. Next, they were inoculated onto plates containing MacConckey Agar and Blood Agar and incubated at 37°C and regularly checked each 24 hours. After the culture, Gram staining smear and identification were performed. ANOVA were performed using SAS.
The predominance of Gram positive bacteria was evident, being the Bacillus sp. the most frequently isolated genus (77.7%). Diplococcus sp. (61.1%), Corynebacterium sp. (50.0%), Streptococcus sp. (38.8%), Staphylococcus sp. (22.2%) and Gram-negative bacilli (16.6%) (Pseudomonas sp. and Aerobacter aerogenes)were also isolated. Micrococcus sp. and Sarcina sp. Were the least commonly (5.5%) isolated microorganisms.
The study revealed the gram-positive aerobic bacteria predominance in brown brocket deer's conjunctiva. Bacillus sp. was present in every examined eye, therefore the most usually isolated genus. Most microorganisms revealed in this study are similar to those present in domestic and wildlife animals, probably by the human contact and urban inhabit (1-3).
The authors would like to thank CAPES for the scholarship provided.
References
1. Rosa A, M. et al. Fungal flora of normal eyes of healthy horses from the State of Rio de Janeiro, Brazil. Vet. Ophthal., v. 6, p.51-55, 2003.
2. Andrade, A. L. et al. Conjunctival microbiota of healthy dogs in Araçatuba city (SP). Arq. Bras. Oftalmol., v.65, p.323-326, 2002.
3. Trindade, R.C. et al. Conjunctival microbial flora of clinically normal person who work in a hospital environment. Braz. J. Microbiol., v.31, p.12-16, 2000.