A retrospective study was carried out to identify reasons and risk factors for limb amputation in cats treated at the University Veterinary Hospital (UVH) of Universiti Putra Malaysia (UPM) from January 2010 to July 2012. A survey using a standardized questionnaire was also conducted by telephone interview to assess the quality of life (QOL) of the feline amputees based on the owners' perception. Clinical data of 43 cats including signalment, reasons for limb amputation and medication given post-surgery were retrieved from the UVH health records. The most common reason for limb amputation in cats treated at UVH was osteomyelitis (58%). Domestic shorthair cat was the only breed treated. Limb amputation was significantly higher in males than female cats; in intact cats and hindlimbs compared to forelimbs. Findings from the QOL survey of 23/43 cat owners revealed that all cats did not show any signs of pain at home despite not having additional or extended prescription for analgesia or inflammation. Approximately 97% of owners informed that their cats returned to routine activity and lead a good quality of life and 87% of these owners would recommend limb amputation if a similar condition occurs to other cats.