Diagnostic imaging is an underutilized resource in exotic pet medicine. Survey radiographs and ultrasound can be used to evaluate many different systems simultaneously, and provide insight into possible problems in a case. To be successful with diagnostic imaging, veterinarians need to acquire a basic knowledge of anatomy regarding the species of interest, methods used to restrain exotic pets to collect the images, and the most appropriate techniques used to collect and interpret the results.

There are a variety of resources available to the veterinarian that is trying to learn more about reptile diagnostic imaging. Expert opinions can be obtained either via phone consults or via the internet (e.g., Veterinary Information Network). There are also board certified radiologists that offer consultation services. Regardless of the source, veterinarians have many options when attempting to interpret the findings of a diagnostic image

With over 60,000 different species of vertebrates, it is impossible to become comfortable with the anatomic peculiarities of all exotic pets. Fortunately, anatomy is highly conserved among the classes of animals. The best way to become comfortable with anatomy of exotic pets is to perform necropsies, read available texts, and practice, practice, practice.

The quality of the equipment used to take radiographs is an important consideration. A radiographic machine to be used for reptiles should be capable of a taking a range of images, which might include day geckos (Phelsuma spp.) to varanids (komodo dragons, Varanus komodoensis). A machine capable of such a range should have a short exposure time. 1/60th of a second or shorter is recommended. The machine should also have a high milliamperes capacity (> 300). This is important because of the variability in detail that might be expected among different sized animals. The kilovolt peak range should also be large, 40–100 kilovolt peak, to accommodate the different sized patients a veterinarian may encounter. The ability to alter the kilovolt peak by small increments (e.g., 2 kilovolt peak) is important because it will enable the veterinarian to review small details between images. A machine in which the tube can be rotated to provide a horizontal beam is preferred. This will enable the veterinarian to take lateral images on animals in sternal recumbency. This is especially important with large chelonians.

For small patient, dental radiograph machines can be used. The author has used this type of equipment to take "whole body" radiographs of small exotic animals (e.g., juvenile bearded dragons, canaries). The detail from these images, although not always refined, does provide more detail than standard films.

Selecting the correct type of cassette or film is as important as using the correct machine. High-detail, rare earth cassettes are preferred. These cassettes should be used in combination with slow speed, single emulsion (gray) films. This combination provides the best detail for small animals. Double emulsion (black) films can be used for larger reptiles when small detail is not required. Selecting the correct size cassette and film combination is also an important consideration.

When taking radiographs it is important to always collect at least two images. The most common images are a lateral and dorsoventral or ventrodorsal image. These two, two-dimensional images will provide the most insight into interpreting the anatomy of a three-dimensional reptile. Care should be taken when positioning an animal to ensure that the area of interest can be evaluated.

An exotic pet must be still to collect the "perfect" image. Taking radiographs on un-anesthetized or restrained animals can lead to motion and a loss of detail. The author has found that exotic pets can be restrained manually for images or anesthetized. Manual restraint does result in increased radiation exposure for the handlers, so it should be kept to a minimum. Placing blinders over the eyes of some animals (e.g., lizard) can also be done to minimize movement. An ophthalmic eye lube is placed in the eyes of the animal and the head is wrapped with vet-wrap (3M products, St. Paul, MN). Dimming the lights and minimizing human movement and speaking in the room will also help reduce the stimuli on the patient.

Interpreting exotic animal radiographs is more challenging than with domestic animals because of a lack of good reference material. Of all the groups, reptiles tend to pose the greatest challenge. Lizards do not store their fat in a mesentery like mammals. This absence of fat between the internal organs reduces the contrast between the tissues, leading the viscera to appear as a single soft tissue structure. Birds, on the other hand, are the easiest group to evaluate because their air sacs provide a good contrast to soft tissues. The absence of a diaphragm also limits the radiographic interpretation of the coelomic structures in some reptiles. The bone of reptiles is less radio opaque than mammals, which can make the interpretation of the cortical densities more difficult. To reduce the likelihood of misclassifying a case of secondary nutritional hyperparathyroidism, the author always evaluates the cortical densities of the long bones instead of the digits. The best way to become comfortable with interpreting radiographs is to practice, practice, and practice.

As veterinarians have become more familiar with ultrasonography, its application in exotic pet medicine has greatly expanded. When considering an ultrasound machine for a veterinary hospital, it is important to consider the range of patients the machine will be used on. If exotic species are going to be regularly screened using ultrasound, a machine with a fine transducer is recommended. The author has found that 10 and 12 mHz transducers are generally best for evaluating exotic pets. Because of the relative small size of many of our exotic pet patients, the author also prefers the transducer to have a small footprint.

Capturing a high detail ultrasound image requires intimate contact between the transducer and the animal. There are two different methods for obtaining high quality ultrasound images: direct contact via a non-irritating coupling gel or direct/non-direct contact via an aquatic medium (e.g., water). Coupling gel is the most common method used for collecting ultrasound images in mammals and birds. In reptiles, this technique can also be used, but it is important to spread the gel between the scales to reduce the loss of detail associated with trapped air bubbles. The transducer can also be placed against a water-filled examination glove that is directed against the reptile's body. This method is the least productive in the author's opinion. Another technique that many reptiles are tolerant of involves placing the animal and the transducer into water. The water acts as an excellent contact medium. The author works with a number of herpetoculturists that use this method for assessing the reproductive status of their reptiles.

Ultrasound can be used to evaluate any number of systems in exotic animals. In reptiles, the author primarily uses ultrasound to assess the reproductive status of an animal. Follicles can be measured to predict whether an animal is likely to ovulate. For some species this information can be used to determine the best time for introducing a male and female. Images of the ovaries are best obtained by placing the transducer on the lateral body wall just caudal to the last rib. Each ovary should be evaluated individually, as one gonad may be active while the other is not. In all species, it can be used to assess for assessing the same systems in domestic mammals. A real benefit of ultrasound is that it allows a veterinarian to collect biopsies in many cases.