Plasma α-Tocopherol and Retinol Levels in Free-Ranging Eastern Painted Turtles (Chrysemys picta picta)
R.A. Cook, VMD; E. Dierenfeld, PhD; T. Norris; J. Behler
Thirty-three wild caught eastern painted turtles (Chrysemys picta picta) were manually restrained for venipuncture via the internal jugular vein. Weights ranged between 143 and 346 g. Whole blood (300-600SI) was collected in plastic Iml syringes coated with heparin sodium using a 26 gauge 19mm needle. Plasma samples were analyzed for vitamin A alltrans retinol and vitamin E (α-tocopherol) using high performance liquid chromatography. The results are listed in Table 1.
Table 1. Plasma vitamin levels (μg/ml) in free-ranging painted turtles (n=33)
|
α- tocopherol
|
all-trans retinol
|
Range
|
1.56-12.64
|
0.07-0.43
|
Mean
|
6.55
|
0. 21
|
S.D.
|
3.04
|
0.08
|