Influence of Habitat in Inner Body Fluids Microbiome in the Small-Spotted Catshark (Scyliorhinus canicula)
IAAAM 2023
Marta Muñoz-Baquero1,4; Laura Lorenzo-Rebenaque1; Francisco A. García-Vázquez2; Daniel García-Párraga3,4; Lucia Martínez-Priego5; Griselda De Marco-Romero5; Inmaculada Galán-Vendrell5; Giuseppe D’Auria5; Francisco Marco-Jiménez6*
1Departamento de Producción y Sanidad Animal, Salud Pública Veterinaria y Ciencia y Tecnología de los Alimentos, Instituto de Ciencias Biomédicas, Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, Alfara del Patriarca, Spain; 2Departamento de Fisiología, Facultad de Veterinaria, Campus de Excelencia Internacional Mare Nostrum, Universidad de Murcia, Murcia, Spain; 3Veterinary Services, Avanqua-Oceanogràfic S.L, Ciudad de las Artes y las Ciencias, Valencia, Spain; 4Research Department Fundación Oceanogràfic de la Comunitat Valenciana, Valencia, Spain; 5Servicio de Secuenciación y Bioinformática, Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunidad Valenciana (FISABIO-Salud Pública), Valencia, Spain; 6Institut of Animal Science and Technology, Universitat Politècnica de València, Valencia, Spain

Abstract

The microbiome is a decisive influencer in the health, development, and/or reproduction of organisms, being an indicator of environmental interactions,1-3 although little is known about the microbial signatures of inner body fluids.4-5 This study compares the environmental influence on the microbial profile found in the peripheral blood and seminal plasma of wild and aquarium small-spotted catshark (Scyliorhinus canicula).

Samples were collected from a total of 18 wild small-spotted catsharks from the Mediterranean Sea and seven aquarium-housed small-spotted catsharks in Oceanogràfic (Ciudad de las Artes y las Ciencias, Valencia, Spain). The study used 16S amplicon sequenced on the Illumina MiSeq. Results were analysed by using QIIME2 v2021.4. The data observed suggest fluid-specific microorganism compartmentalisation in both inner body fluids. The blood plasma microbiome was not affected by the environment because there are no differences in the α- and β-diversity between groups, in contrast with seminal plasma. Proteobacteria dominated the bacterial communities in blood and seminal plasma from both environments, and Firmicutes was only present in the blood plasma of wild animals. No central microbiome was identified at the genus level; only Pseudomonas and Cloacibacterium appeared as common genera in both body fluids and habitats. Fourteen genera were identified in blood plasma, but only four were common in wild and aquarium S. canicula, in contrast with 100 genera identified in seminal plasma, only 41 being shared between groups.

Altogether, this study extends the existing knowledge about the elasmobranchs’ microbiota under wild and aquaria conditions differing from environmental factors; meanwhile, providing further evidence that each body compartment has a unique microbiome and suggesting that their blood microbiomes are not associated with compromised host health.6

Acknowledgments

The authors are grateful to the Avanqua-Oceanogràfic SL aquarium staff, especially to the aquarists and veterinarians for their assistance with animal care in the aquarium animals, and to the local fishermen from Valencia, Jávea, and Cullera for providing wild-captured animals.

*Presenting author

Literature Cited

1.  Perry CT, Pratte ZA, Clavere-Graciette A, Ritchie KB, Hueter RE, Newton AL, et al. Elasmobranch microbiomes: emerging patterns and implications for host health and ecology. Anim Microbiome. 2021;3:61.

2.  Patin NV, Pratte ZA, Regensburger M, et al. Microbiome dynamics in a large artificial seawater aquarium. Appl Environ Microbiol. 2018;84(10):e0017918.

3.  Clavere-Graciette AG, McWhirt ME, Hoopes LA, et al. Microbiome differences between wild and aquarium white-spotted eagle rays (Aetobatus narinari). Anim Microbiome. 2022;4:34.

4.  Doane MP, Haggerty JM, Kacev D, Papudeshi B, Dinsdale EA. The skin microbiome of the common thresher shark (Alopias vulpinus) has low taxonomic and gene function β-diversity: skin microbiome of the common thresher shark. Environ Microbiol Rep. 2017;9:357–373.

5.  Pogoreutz C, Gore MA, Perna G, et al. Similar bacterial communities on healthy and injured skin of black tip reef sharks. Anim Microbiome. 2019;1:9.

6.  Mylniczenko ND, Harris B, Wilborn RE, Young FA. Blood culture results from healthy captive and free-ranging elasmobranchs. J Aquat Anim Health. 2007;19:159–167.

 

 

Speaker Information
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Marta Muñoz-Baquero
Departamento de Producción y Sanidad Animal
Salud Pública Veterinaria y Ciencia y Tecnología de los Alimentos
Instituto de Ciencias Biomédicas, Facultad de Veterinaria
Universidad Cardenal Herrera-CEU
Alfara del Patriarca, Spain


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