E. rhusiopathiae is the etiological agent of serious diseases in domestic and wild animals, fowl, and cetaceans. Agglutinins have usually been used to detect antibodies. This report describes a more sensitive assay (ELISA) for detection of antibodies in humans and marine mammals.

Six serotypes have been isolated from pinnipeds and wounds of humans bitten at the CMMC. Using specific anti-IgG, antibody titers were determined in 6 humans of 19 who were bitten and developed 'seal finger', 22 people who were bitten but did not develop lesions, 68 who worked with animals but were not bitten, and dolphins following vaccination.

The isolation techniques, cell component used in the assay and future areas of research will be discussed.