Edwardsiella ictaluri is the etiologic agent of Enteric Septicemia of Catfish, an infectious disease of major economic significance to the commercial catfish industry in Mississippi Channel catfish fingerlings were challenged with 10⁴, 10⁵, and 10⁶ bacteria (IP) and mortalities as well as clinical data were recorded for 10 days. Control fish were injected with sterile saline. Trials were run at 6 water temperatures, 17°, 21°, 23°, 25°, 28°, and 32°C. Disease and significant mortality occurred when water tempatures were between 22° and 28° C which correlates with field observations of natural infections. Cumulative mortality, calculated LD, and a brief clinical description of the disease will be presented with appropriate recommendations for management and prevention.