Ten dogs were vaccinated using an experimental vaccine containing recombinant OspA and OspC proteins of serospecies Borrelia burgdorferi, Borrelia afzelii and Borrelia garinii. Two dogs without vaccination were used as controls. A challenge test was accomplished using the natural way of contact (with infected common ticks - Ixodes ricinus). Blood samples for serological examination were collected after the immunisation prior to and after the challenge. An autopsy was performed 2 months after the challenge and samples were collected for the culture detection of borrelia from the predilection organs. Culture detection was performed also after the challenge prior to autopsy from biopsy skin samples. It was verified that the experimental recombinant vaccine induces specific antibodies against OspA and OspC proteins and results in protection against borrelia during natural infection from common ticks - Ixodes ricinus. Results from project of MPO - FD-K3/100 were used in the presentation.